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amersham hybridization solution  (Amersham Life Sciences Inc)

 
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    Amersham Life Sciences Inc amersham hybridization solution
    Amersham Hybridization Solution, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amersham+hybridization+solution/hybridization+rapid+solution/pm16825954-45-29-32
    Average 86 stars, based on 1 article reviews
    amersham hybridization solution - by Bioz Stars, 2026-10
    86/100 stars

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    Hybridization:

    Article Title: Microarray analysis reveals differential gene expression patterns in tumors of the pineal region.
    Article Snippet: Amplified RNA (aRNA) was measured with an ultraviolet spectrophotometer and the quality verified on picochips using the Agilent bioanalyzer. .. Biotin-labeled aRNA (10Kg) was fragmented with 5 KL of fragmentation buffer (GE Healthcare, Amersham, Saclay, France) in a final volume of 20 KL and the fragmented aRNA added to Amersham hybridization solution (Amersham) (final volume 260 KL) and injected onto CodeLink Uniset human 20K bioarrays containing 19,000 human oligonucleotide gene probes (Amersham). .. The arrays were hybridized overnight at 37-C at 300 rpm on a rotary mixer in an incubator, washed at 46-C for 1 hour in stringent TNT buffer (100 mM Tris, 150 mM NaCl, 0.02% Tween 20; all from SigmaAldrich, Saint Quentin-Fallavier, France), incubated in 3.4 mL of streptavidin-Cy5 (Amersham) solution for 30 minutes, washed 4 times in 240 mL of TNT buffer, rinsed twice in 240 mL of water containing 0.2% Triton X-100, and dried by centrifugation at 600 rpm.

    Injection:

    Article Title: Microarray analysis reveals differential gene expression patterns in tumors of the pineal region.
    Article Snippet: Amplified RNA (aRNA) was measured with an ultraviolet spectrophotometer and the quality verified on picochips using the Agilent bioanalyzer. .. Biotin-labeled aRNA (10Kg) was fragmented with 5 KL of fragmentation buffer (GE Healthcare, Amersham, Saclay, France) in a final volume of 20 KL and the fragmented aRNA added to Amersham hybridization solution (Amersham) (final volume 260 KL) and injected onto CodeLink Uniset human 20K bioarrays containing 19,000 human oligonucleotide gene probes (Amersham). .. The arrays were hybridized overnight at 37-C at 300 rpm on a rotary mixer in an incubator, washed at 46-C for 1 hour in stringent TNT buffer (100 mM Tris, 150 mM NaCl, 0.02% Tween 20; all from SigmaAldrich, Saint Quentin-Fallavier, France), incubated in 3.4 mL of streptavidin-Cy5 (Amersham) solution for 30 minutes, washed 4 times in 240 mL of TNT buffer, rinsed twice in 240 mL of water containing 0.2% Triton X-100, and dried by centrifugation at 600 rpm.



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